Protein analysis
Glycan
Innovative Technology Support Group for Life Science and Drug Discovery
PC6-1 : N-Glycan Structure Analysis
Test Introduction
Oligosaccharide population analysis from PROTEINWORKS, oligosaccharide analysis to help you identify the overall population profile
of glycans present in a glycoprotein. Oligosaccharide population analysis is performed following release of the glycans from the supplied
product using the following techniques. Determination of structure and relative quantity of glycan in target proteins using MALDI-TOF MS
after steps of enrichment and GT derivatization of N-Glycan.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Glycosidase Digestion
- Derivatization
- MALDI-TOF MS
- N-glycan profile analysis
Quantitative analysis
Analysis Result
GT-Labeled
Composition
Proposed structure
m/z
Intensity
Percentage total
Found[M]+
Calculated[M]+
Hex
HexNAc
dHex(NeuAc)
1348.5
1348.5
5
2
0(0)
13483.36
3909
9.450
1389.6
1389.6
4
3
0(0)
1389.38
645
1.559
1510.5
1510.5
6
2
0(0)
1510.39
4549
10.999
1592.6
1592.6
4
4
0(0)
1592.44
3840
9.284
1672.5
1672.5
7
2
0(0)
1672343
1855
4.484
1738.6
1738.7
4
4
1(0)
1738.48
328
0.787
1754.6
1754.7
5
4
0(0)
1754.48
5804
14.033
1834.6
1834.6
8
2
0(0)
1894.46
1545
3.736
1900.7
1900.7
5
4
1(0)
1900.52
7036
17.012
1996.7
1996.7
9
2
0(0)
1996.50
1779
4.302
2043.2
2043.7
4
4
1(1)
2043.55
101
0.245
2059.8
2059.7
5
4
0(1)
2059.56
1299
3.140
2205.8
2205.7
5
4
1(1)
2205.60
7193
17.392
2265.9
2265.9
6
5
1(0)
2265.62
196
0.473
2511.0
2510.8
5
4
1(2)
2511.66
838
2.026
2571.0
2570.9
6
5
1(1)
2571.67
323
0.781
2876.2
2876.0
6
5
1(2)
2876.72
86
0.208
2936.2
2936.0
7
6
1(1)
2936.71
36
0.087
41359
100
Test Introduction
Oligosaccharide population analysis from PROTEINWORKS, oligosaccharide analysis to help you identify the overall population profile of glycans present in a glycoprotein. Oligosaccharide population analysis is performed following release of the glycans from the supplied product using the following techniques. Determination of structure and relative quantity of glycan in target proteins using MALDI-TOF MS after steps of enrichment and GT derivatization of N-Glycan.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Glycosidase Digestion
- Derivatization
- MALDI-TOF MS
- N-glycan profile analysis
Quantitative analysis
Analysis Result
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| GT-Labeled | Composition | Proposed structure | m/z | Intensity | Percentage total | |||
|---|---|---|---|---|---|---|---|---|
| Found[M]+ | Calculated[M]+ | Hex | HexNAc | dHex(NeuAc) | ||||
| 1348.5 | 1348.5 | 5 | 2 | 0(0) | |
13483.36 | 3909 | 9.450 |
| 1389.6 | 1389.6 | 4 | 3 | 0(0) | |
1389.38 | 645 | 1.559 |
| 1510.5 | 1510.5 | 6 | 2 | 0(0) | |
1510.39 | 4549 | 10.999 |
| 1592.6 | 1592.6 | 4 | 4 | 0(0) | |
1592.44 | 3840 | 9.284 |
| 1672.5 | 1672.5 | 7 | 2 | 0(0) | |
1672343 | 1855 | 4.484 |
| 1738.6 | 1738.7 | 4 | 4 | 1(0) | |
1738.48 | 328 | 0.787 |
| 1754.6 | 1754.7 | 5 | 4 | 0(0) | |
1754.48 | 5804 | 14.033 |
| 1834.6 | 1834.6 | 8 | 2 | 0(0) | |
1894.46 | 1545 | 3.736 |
| 1900.7 | 1900.7 | 5 | 4 | 1(0) | |
1900.52 | 7036 | 17.012 |
| 1996.7 | 1996.7 | 9 | 2 | 0(0) | |
1996.50 | 1779 | 4.302 |
| 2043.2 | 2043.7 | 4 | 4 | 1(1) | |
2043.55 | 101 | 0.245 |
| 2059.8 | 2059.7 | 5 | 4 | 0(1) | |
2059.56 | 1299 | 3.140 |
| 2205.8 | 2205.7 | 5 | 4 | 1(1) | |
2205.60 | 7193 | 17.392 |
| 2265.9 | 2265.9 | 6 | 5 | 1(0) | |
2265.62 | 196 | 0.473 |
| 2511.0 | 2510.8 | 5 | 4 | 1(2) | |
2511.66 | 838 | 2.026 |
| 2571.0 | 2570.9 | 6 | 5 | 1(1) | |
2571.67 | 323 | 0.781 |
| 2876.2 | 2876.0 | 6 | 5 | 1(2) | |
2876.72 | 86 | 0.208 |
| 2936.2 | 2936.0 | 7 | 6 | 1(1) | |
2936.71 | 36 | 0.087 | 41359 | 100 |
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