Epitope Mapping

EP-101 : Epitope Mapping

Test Introduction

Epitope is an antigenic determinant which is a structure recognized by an antibody. To confirm the binding position of the antigen and the antibody three analytical methods are performed. Generally, analysis methods are consist of Covalent labeling, Cross Link, Immuno-affinity peptide screening. Three types of analyses are used to determine the linear and conformational epitope positions of the antigen protein.

Epitope Mapping Process

Sample

  • Biosimilar
  • Biobetter
  • Antibody
  • Protein-protein interaction

Analysis Process

  • Ag-Ab complex
  • 1) DEPC labeling
    2) Chemical cross linking
  • Protease Digestion
  • LC-MS
    LC-MS/MS
  • Epitope Map

  • Antigen
  • Protease Digestion
  • Immuno-affinity
    biding assay
  • LC-MS
    LC-MS/MS
  • Epitope Map

Analysis Result

1) DEPC labeling

2) Chemical cross link

3) Immuno-affinity peptide screening

좌우로 스크롤하여 내용을 확인하세요.
Unbinding fraction Elution fraction (Area) sequence
Frag# Res Area 1st 2nd 3rd
T14 180-188 1,291,000,000 810,600 8,799,000 11,950,000 TAAGGPVTSR
T16 235-243 799,700,000 2,995,500 3,023,000 2,819,000 VDDPPQNGDNDR
T21 281-288 66,900,000 1,425,000 1,232,000 1,367,000 WQCQLYQGER

Conclusion