mRNA analysis

mRNA Analysis

Inovative Technology Support Group for Life Science and Drug discovery

mRNA Analysis
5’ Cap

5’ Cap Analysis

Test Introduction

Efficient translation of mRNA to protein depends critically on a 5'-terminal dinucleotide modification called capping which is appended to mRNA during or after the transcription process. Importantly, the percentage of successfully capped material and the type of capping structure appended is influenced by factors such as the quality of input materials, the reaction conditions, and the mRNA sequence. mRNA 5' capping is therefore a critical quality attribute that should be thoroughly characterized and monitored.

Sample

  • Raw mRNA pharmaceuticals
  • Final mRNA pharmaceuticals

Analysis Process

  • mRNA
  • Annealing &
    cleavage probe
  • Binding to beads and
    RNase H cleavage
  • Cap analysis and
    quantitative analysis
    by LC-MS

Analysis Result