Protein analysis
Glycan
Innovative Technology Support Group for Life Science and Drug Discovery
PC4-1 : N-Glycosylation Site Determination
Test Introduction
Glycosylation sites are initially identified as part of a peptide mapping study using Liquid chromatography–mass spectrometry (LCMS).
Glycosylation at each individual site is then characterized following release of the glycans from each purified glycopeptide using a
range of effective techniques, including fragment ion information from LC-MS/MS. It is one of the process of peptide mapping involving
enzymes treatment (PNGase-F and protease) for determining the localization of oligosaccharide linked to asparagine in target proteins.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Protease Digestion
PNGase-F
- LC-MS, LC-MS/MS
- De-novo sequencing
- Data interpretation
Analysis Result
De-glycosylated peptide sequencing
Test Introduction
Glycosylation sites are initially identified as part of a peptide mapping study using Liquid chromatography–mass spectrometry (LCMS). Glycosylation at each individual site is then characterized following release of the glycans from each purified glycopeptide using a range of effective techniques, including fragment ion information from LC-MS/MS. It is one of the process of peptide mapping involving enzymes treatment (PNGase-F and protease) for determining the localization of oligosaccharide linked to asparagine in target proteins.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Protease Digestion
PNGase-F - LC-MS, LC-MS/MS
- De-novo sequencing
- Data interpretation
Analysis Result
De-glycosylated peptide sequencing
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