Protein analysis

Proteomics & Electrophoresis

Innovative Technology Support Group for Life Science and Drug Discovery

Proteomics
MS1
Electrophoresis
ELECTRO-1

MS3 : Total proteome identification by Nano-LC-MS/MS

Test Introduction

MS3 is a test method to protein identification from total proteome sample by the action of protease and identification of total protein through automatic LC-MS/MS analysis. Analysis procedure is to prepare protein-based peptide using In-gel digestion (In-solution digestion) and then identification of total protein by using Automatic LC-MS/MS or De-novo sequencing

Sample

  • Proteomic sample.

Analysis Process

  • In-Solution digestion
  • Automatic LC-MS/MS
  • Database search

Analysis Result

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Accession Score Mass Matches Match(sig) Sequences Description
BDLA_ASPNC 460 93571 28 28 12 Annexine II
AMG_ASPAW 225 68837 13 13 7 Beta-glucoamylase  
AXHA_ASPNC 151 35928 5 5 2 Lipase
NLTP1_WHEAT 141 12347 8 8 4 Non-specific lipid-transfer protein (Fragment) 
ABFB_ASPAW 127 53070 6 6 3 Probable alpha-L-arabinofuranosidase B  
XYND_ASPAW 120 87656 6 6 3 Exo-1,4-beta-xylosidase xlnD
RN28_PANGI 116 27665 2 2 1 Ribonuclease -like storage protein  
CBHA_ASPNC 102 49242 7 7 3 Probable 1,4-beta-D-glucan cellobiohydrolase A  
PEPA_ASPAW 82 41266 5 5 4 Aspartic protease pep1  
CBHC_ASPNC 70 48723 2 2 1 Probable 1,4-beta-D-glucan cellobiohydrolase C