Protein analysis

Proteomics & Electrophoresis

Innovative Technology Support Group for Life Science and Drug Discovery

Proteomics
MS1
Electrophoresis
ELECTRO-1

MS4 : Total proteome identification & quantitative analysis by Nano-LC-MS/MS

Test Introduction

MS4 is a test method to protein composition analysis from total proteome by the action of the protease and LC-MS/MS. The protein-based peptide is then total protein identification through Automatic LC-MS/MS methods and also analysis total contents of identifying protein. The analysis procedure is to get results from automatic LC-MS/MS using protein-based peptide with In-gel digestion (In-solution digestion) method and then analyze each protein contents in total proteome by confirming identify peptide from Mascot Database.

Sample

  • Proteomic sample
  • Protein Sample

Analysis Process

  • In-Solution digestion
  • Automatic LC-MS/MS
  • Database search
  • Protein composition

Analysis Result

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Accession Score Mass Matches Match(sig) Sequences Seq(sig) emPAI Description Protein content (weight %)
BDLA_ASPNC 460 93571 28 28 12 12 0.42 Annexine II 32.54
AMG_ASPAW 225 68837 13 13 7 7 0.32 Beta-glucoamylase   18.24
AXHA_ASPNC 151 35928 5 5 2 2 0.16 Lipase 4.76
NLTP1_WHEAT 141 12347 8 8 4 4 1.3 Non-specific lipid-transfer protein (Fragment)  13.29
ABFB_ASPAW 127 53070 6 6 3 3 0.16 Probable alpha-L-arabinofuranosidase B   7.03
XYND_ASPAW 120 87656 6 6 3 3 0.1 Exo-1,4-beta-xylosidase xlnD   7.26
RN28_PANGI 116 27665 2 2 1 1 0.1 Ribonuclease-like storage protein   2.29
CBHA_ASPNC 102 49242 7 7 3 3 0.18 Probable 1,4-beta-D-glucan cellobiohydrolase A   7.34
CBHC_ASPNC 70 48723 2 2 1 1 0.06 Probable 1,4-beta-D-glucan cellobiohydrolase C   2.42
ABNC_ASPNC 65 34311 3 3 2 2 0.17 Probable arabinan endo-1,5-alpha-L-arabinosidase C   4.83