Extinction Coefficient(EC)
Capillary Electrophoresis
Liquid chromatography(LC)

Liquid chromatography(LC) : IEX-HPLC (Ion Exchange HPLC)

Test Introduction

HPLC-3 is a test item for analyzing charge isoform of biomedicine. Confirm the contents of charge variant in refined protein/peptide by using Ion Exchange-HPLC analysis method.

Sample

  • Biosimilar
  • Biobetter
  • Antibody
  • Antibody-drug conjugates
  • Recombinant protein drug
  • Toxine
  • vaccine

Analysis Process

  • Protein sample
  • IEX-HPLC
  • Quantitative analysis
    Data interpretation

Analysis Result

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PROTEINWORKS (UV280nm)
No. Time Area Area%
1 3.8 9295 14.0
2 4.2 12865 19.4
3 5.7 22739 34.2
4 8.0 21500 32.4