Protein analysis

Protein

Innovative Technology Support Group for Life Science and Drug Discovery

PC2-2

PC2-2 : C-term sequencing by LC-ESI-MS/MS

Test Introduction

PC2-2 is a test method for analyzing C-terminal of protein sequencing using a mass spectrometer. C-terminal of protein is a structure where can be modified in various ways. Using protein-digesting enzymes, protease, confirm the sequencing of C-terminal peptide which consists of 3 ~ 20 types of amino acid through mass spectrometer. It has been known that Lysine truncation or Drug conjugate is one of major C-terminal modification in Glutamine amino acid.

Sample

  • Biosimilar
  • Biobetter
  • Antibody
  • Antibody-drug conjugates
  • Recombinant protein drug
  • Toxin
  • vaccine

Analysis Process

  • Protease Digestion
  • LC-MS, LC-MS/MS
  • Characterization of peaks
    De-novo sequencing
  • Determination of
    C-terminal sequence and modification

Analysis Result

N-term sequencing