Protein analysis
Protein
Innovative Technology Support Group for Life Science and Drug Discovery
PC5-2 : Amino acid Composition Analysis
Test Introduction
Amino acid composition analysis is a vital tool at all stages of drug discovery and throughout the manufacturing process. Composition of
amino acids in target proteins is to evaluate the quantities of each amino acid in target proteins after hydrolysis of proteins. Amino acid
ratio can be determined by comparing data of amino acid obtained by LC to theoretical ratio of amino acids.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Acid Hydrolysis of Amino Acid
- AccQ Tag Derivatization
- HPLC separation
- Amino Acid Composition
Analysis Result
Edman cycle
Scroll to the left and right to reveal the contents.
A.A
Standard(%)
Sample (%)
Theoretical amino acid
a.a
%
Cys
2.88
2.33
5
2.87
Asp
1.56
2.40
4
2.30
Glu
15.41
17.40
26
14.94
Ser
3.79
4.49
14
8.05
Gly
3.94
9.04
14
8.05
His
1.62
2.23
5
2.87
Arg
9.67
4.63
5
2.87
Thr
2.60
2.99
7
4.02
Ala
6.22
7.72
19
10.92
Pro
6.20
10.04
13
7.47
Tyr
1.96
2.05
3
1.72
Val
3.84
3.44
7
4.02
Met
3.10
1.48
3
1.72
Ile
2.56
2.25
4
2.30
Leu
16.12
16.65
33
18.97
Phe
8.40
3.95
6
3.45
Trp
1.32
1.10
2
1.15
Lys
8.82
5.82
4
2.30
TOTAL
100
100
174
100.00
Test Introduction
Amino acid composition analysis is a vital tool at all stages of drug discovery and throughout the manufacturing process. Composition of amino acids in target proteins is to evaluate the quantities of each amino acid in target proteins after hydrolysis of proteins. Amino acid ratio can be determined by comparing data of amino acid obtained by LC to theoretical ratio of amino acids.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Acid Hydrolysis of Amino Acid
- AccQ Tag Derivatization
- HPLC separation
- Amino Acid Composition
Analysis Result
Edman cycle
Scroll to the left and right to reveal the contents.
| A.A | Standard(%) | Sample (%) | Theoretical amino acid | |
|---|---|---|---|---|
| a.a | % | |||
| Cys | 2.88 | 2.33 | 5 | 2.87 |
| Asp | 1.56 | 2.40 | 4 | 2.30 |
| Glu | 15.41 | 17.40 | 26 | 14.94 |
| Ser | 3.79 | 4.49 | 14 | 8.05 |
| Gly | 3.94 | 9.04 | 14 | 8.05 |
| His | 1.62 | 2.23 | 5 | 2.87 |
| Arg | 9.67 | 4.63 | 5 | 2.87 |
| Thr | 2.60 | 2.99 | 7 | 4.02 |
| Ala | 6.22 | 7.72 | 19 | 10.92 |
| Pro | 6.20 | 10.04 | 13 | 7.47 |
| Tyr | 1.96 | 2.05 | 3 | 1.72 |
| Val | 3.84 | 3.44 | 7 | 4.02 |
| Met | 3.10 | 1.48 | 3 | 1.72 |
| Ile | 2.56 | 2.25 | 4 | 2.30 |
| Leu | 16.12 | 16.65 | 33 | 18.97 |
| Phe | 8.40 | 3.95 | 6 | 3.45 |
| Trp | 1.32 | 1.10 | 2 | 1.15 |
| Lys | 8.82 | 5.82 | 4 | 2.30 |
| TOTAL | 100 | 100 | 174 | 100.00 |
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