PC3-1 : Disulfide Bridge Determination

Test Introduction

Disulfide bridge analysis by mass spectrometry, a powerful technique to help you identify and quantify disulfide bridges. Determination of localization of disulfide bond and free sulfydryl group in target proteins using LC-MS, LC-MS/MS after enzyme/DTT treatment steps. Our services help to detect potential mismatched or scrambled disulfide bridge forms using powerful mass spectrometric techniques.

Sample

  • Biosimilar
  • Biobetter
  • Antibody
  • Antibody-drug conjugates
  • Recombinant protein drug
  • Toxin
  • vaccine

Analysis Process

  • Protease Digestion
  • LC-MS, LC-MS/MS
  • Characterization of peaks and
    De-novo sequencing
  • Determination of S-S bonds

Analysis Result