Protein analysis
Glycan
Innovative Technology Support Group for Life Science and Drug Discovery
PC4-2 : O-Glycosylation Site Determination and O-Glycan Structure Analysis
Test Introduction
PC4-2 is a test method to analysis glycosylation site of glycoprotein through peptide map and De-novo sequencing. It is defined by confirming glycosylation of Serine and Theronine from amino acid in protein. Unlike N-linked glycosylation, O-linked glycosylation known to compose of complicated heterogenous structure and did not break down by the action of glycosidase. Therefore, O-linked glycosylation analysis methods
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Protease Digestion
- LC-MS, LC-MS/MS
- De-novo sequencing
- Quantitative analysis
Data interpretation
Analysis Result
Test Introduction
PC4-2 is a test method to analysis glycosylation site of glycoprotein through peptide map and De-novo sequencing. It is defined by confirming glycosylation of Serine and Theronine from amino acid in protein. Unlike N-linked glycosylation, O-linked glycosylation known to compose of complicated heterogenous structure and did not break down by the action of glycosidase. Therefore, O-linked glycosylation analysis methods
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Protease Digestion
- LC-MS, LC-MS/MS
- De-novo sequencing
- Quantitative analysis
Data interpretation
Analysis Result
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