Protein analysis

Glycan

Innovative Technology Support Group for Life Science and Drug Discovery

PC4-1

PC6-3 : Sialic acid Composition Analysis

Test Introduction

A powerful technique to help you identify and quantify sialic acid species in your biopharmaceutical product. Determination of quantity/ composition of sialic acid in glycoproteins which can play an important role in protein activity and immunogenesity using HPAEC-PAD (High Performance Anion Exchange Chromatography-Pulsed Amperometry Detection) method after weak acid hydrolysis of glycoproteins.

Sample

  • Biosimilar
  • Biobetter
  • Antibody
  • Antibody-drug conjugates
  • Recombinant protein drug
  • Toxin
  • vaccine

Analysis Process

  • Acid Hydrolysis
  • Bio-LC
  • Characterization of peaks
  • Quantitative analysis

Analysis Result

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Samples/sugars Recombinant Protein
fucose 2.5
galactose 5.2
mannose 17.0
galactosamine 0.5
glucosamine 9.4
N-acetylneuraminic acid 2.1
N-glycolylneuraminic acid 0.1
Sugar (%, w/w) 3.7
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No. Ret.Time min Peak Name Cal.Type Points Corr.Coeff. Offset Slope Curve
1 3.27 NANA Lin 3 99.7848 0.0000 0.0164 0.0000
2 8.53 NGNA Lin 3 99.6355 0.0000 0.0457 0.0000
Average: 99.7102 0.0000 0.0310 0.0000
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No. Ret.Time min Peak Name Cal.Type Points Corr.Coeff. Offset Slope Curve
1 3.02 N-Acetylneuraminic Lin 3 99.7848 0.0000 0.0164 0.0000
2 8.53 NGNA Lin 3 99.6355 0.0000 0.0457 0.0000
Average: 99.7102 0.0000 0.0310 0.0000