Protein analysis
Glycan
Innovative Technology Support Group for Life Science and Drug Discovery
PC6-1 : Site-specific N-Glycan Profile Analysis
Test Introduction
PC6-2 is a test method for N-glycan structure analysis of glycoprotein. It defines a structure of glycan and glycan structure that linked each site of amino acids. In a case of glycoprotein that has more than two glycosylation sites, then it is possible to reveal details of the glycan of protein by confirming the structure of glycan in each glycosylation site.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Protease Digestion
- LC-MS, LC-MS/MS
- Characterization
of peaks
- Site-specific N-glycan profile
Data interpretation
Analysis Result
Scroll to the left and right to reveal the contents.
Sample
Modifiers
Theoretical mass
Obserced mass(Da)
RT(Min)
Intensity(Counts)
Error(ppm)
%
Modified
PROTEINWORKS
G1F+1SA
2962.172
2962.181
11.3
3708
3.2
0.55
G2F+1SA
3124.172
3124.205
11.2
90396
10.7
13.33
G2F+2SA
3415.266
3415.307
11.3
270356
11.9
39.85
G3F
3198.208
3198.245
10.8
6548
11.7
0.97
G3F+1SA
3489.303
3489.356
11.0
31584
11.7
0.97
G3F+2SA
3780.398
3780.437
11.1
71436
10.5
10.53
G3F+3SA
4071.492
4071.533
11.5
66532
10.0
9.81
G4F+1SA
3854.434
3854.432
10.9
13188
-0.4
1.94
G4F+2SA
4145.529
4145.564
10.9
33468
8.6
4.93
G4F+3SA
4436.624
4436.675
11.1
41956
11.6
6.18
G4F+1LN+2SA
4510.660
4510.679
10.7
11920
4.3
1.76
G4F+1LN+3SA
4801.755
4801.811
10.8
20856
11.8
3.07
G4F+2LN+2SA
4875.791
4875.758
10.7
8500
-6.7
1.25
G4F+2LN+3SA
5166.886
5166.941
10.7
7912
10.7
1.17
Test Introduction
PC6-2 is a test method for N-glycan structure analysis of glycoprotein. It defines a structure of glycan and glycan structure that linked each site of amino acids. In a case of glycoprotein that has more than two glycosylation sites, then it is possible to reveal details of the glycan of protein by confirming the structure of glycan in each glycosylation site.
Sample
- Biosimilar
- Biobetter
- Antibody
- Antibody-drug conjugates
- Recombinant protein drug
- Toxin
- vaccine
Analysis Process
- Protease Digestion
- LC-MS, LC-MS/MS
- Characterization
of peaks - Site-specific N-glycan profile
Data interpretation
Analysis Result
Scroll to the left and right to reveal the contents.
| Sample | Modifiers | Theoretical mass | Obserced mass(Da) | RT(Min) | Intensity(Counts) | Error(ppm) | % Modified |
|---|---|---|---|---|---|---|---|
| PROTEINWORKS | G1F+1SA | 2962.172 | 2962.181 | 11.3 | 3708 | 3.2 | 0.55 |
| G2F+1SA | 3124.172 | 3124.205 | 11.2 | 90396 | 10.7 | 13.33 | |
| G2F+2SA | 3415.266 | 3415.307 | 11.3 | 270356 | 11.9 | 39.85 | |
| G3F | 3198.208 | 3198.245 | 10.8 | 6548 | 11.7 | 0.97 | |
| G3F+1SA | 3489.303 | 3489.356 | 11.0 | 31584 | 11.7 | 0.97 | |
| G3F+2SA | 3780.398 | 3780.437 | 11.1 | 71436 | 10.5 | 10.53 | |
| G3F+3SA | 4071.492 | 4071.533 | 11.5 | 66532 | 10.0 | 9.81 | |
| G4F+1SA | 3854.434 | 3854.432 | 10.9 | 13188 | -0.4 | 1.94 | |
| G4F+2SA | 4145.529 | 4145.564 | 10.9 | 33468 | 8.6 | 4.93 | |
| G4F+3SA | 4436.624 | 4436.675 | 11.1 | 41956 | 11.6 | 6.18 | |
| G4F+1LN+2SA | 4510.660 | 4510.679 | 10.7 | 11920 | 4.3 | 1.76 | |
| G4F+1LN+3SA | 4801.755 | 4801.811 | 10.8 | 20856 | 11.8 | 3.07 | |
| G4F+2LN+2SA | 4875.791 | 4875.758 | 10.7 | 8500 | -6.7 | 1.25 | |
| G4F+2LN+3SA | 5166.886 | 5166.941 | 10.7 | 7912 | 10.7 | 1.17 |
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