Protein analysis

Glycan

Innovative Technology Support Group for Life Science and Drug Discovery

PC4-1

PC6-4 : RapiFluor labeled N-Glycan Analysis by HILIC-UPLC/ ESI-MS

Test Introduction

Determination of antennary N-glycan profiling (included structure and quantity of glycan) linked to glycoproteins using HILIC (Hydrophilic Interaction Liquid Chromatography) and ESI-MS methods after RapiFluor derivatization of glycan from target glycoproteins.

Sample

  • Biosimilar
  • Biobetter
  • Antibody
  • Antibody-drug conjugates
  • Recombinant protein drug
  • Toxin
  • vaccine

Analysis Process

  • Glycosidase Digestion
  • Labeling
  • UPLC, ESI-MS
    Characterization of peaks
  • Quantitative analysis

Analysis Result

  • HILIC chromatogram RSD : 4.45 (20개 HILIC peak 기준)
  • Average Mass Accuracy : 6.14 ppm
Scroll to the left and right to reveal the contents.
Sample Peak Number (% Area)
PROTEIN1 5.553 0.064 1.092 2.840 10.308 3.128 7.839 10.980 7.750 10.760 21.557 18.130
PROTEIN2 5.586 0.316 0.278 1.603 10.331 1.853 7.711 11.976 7.837 10.551 22.408 19.551
Glycan structure G2F+ 1SA G4F+1LN+2SA G4F+2SA G3F+2SA G2F+2SA G4F+2LN +3SA G4F+1LN+3SA G4F+3SA G3F+3SA G4F+2LN+4SA G4F+1LN+4SA G4F+ 4SA